MersV1, Main, Exploration, bibRecord, 002B76

A randomized lentivirus shRNA library construction.

Identifieur interne : 002B76 ( Main/Exploration ); précédent : 002B75; suivant : 002B77

A randomized lentivirus shRNA library construction.

Auteurs : Qiang Guo [République populaire de Chine] ; Yirong Kong ; Ling Fu ; Ting Yu ; Junjie Xu ; Wei Chen

Source :

Biochemical and biophysical research communications [ 0006-291X ] ; 2007.

RBID : pubmed:17482565

Descripteurs français

KwdFr :
- ADN viral (synthèse chimique), ARN (génétique), ARN (métabolisme), ARN viral (génétique), Animaux, Banque de gènes, Cellules CHO, Clonage moléculaire, Cricetinae, Cricetulus, Humains, Interférence par ARN, Lentivirus (génétique), Régions promotrices (génétique).
MESH :
- génétique : ARN, ARN viral, Lentivirus.
- métabolisme : ARN.
- synthèse chimique : ADN viral.
- Animaux, Banque de gènes, Cellules CHO, Clonage moléculaire, Cricetinae, Cricetulus, Humains, Interférence par ARN, Régions promotrices (génétique).

English descriptors

KwdEn :
- Animals, CHO Cells, Cloning, Molecular, Cricetinae, Cricetulus, DNA, Viral (chemical synthesis), Gene Library, Humans, Lentivirus (genetics), Promoter Regions, Genetic, RNA (genetics), RNA (metabolism), RNA Interference, RNA, Viral (genetics).
MESH :
- chemical , chemical synthesis : DNA, Viral.
- genetics : Lentivirus, RNA, RNA, Viral.
- chemical , metabolism : RNA.
- Animals, CHO Cells, Cloning, Molecular, Cricetinae, Cricetulus, Gene Library, Humans, Promoter Regions, Genetic, RNA Interference.

Abstract

Vector based shRNA (short hairpin RNA) expression library has been widely used to screen functional genes. For two main methods that have been used to generate short hairpin RNA libraries, chemical synthesis is too expensive to be widely used and the low efficiency of enzymatic approach makes it difficult to construct. We have developed a protocol to construct a new kind of shRNA library called randomized shRNA library. Within three steps chemically synthesized randomized 19-mers DNA were efficiently converted to double-stranded DNA fragments containing shRNA templates. This kind of shRNA library permits simple and economic construction, providing another choice for whole-genome phenotypic screening of genes.

DOI: 10.1016/j.bbrc.2007.04.123
PubMed: 17482565

Affiliations:

Le document en format XML

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<author><name sortKey="Xu, Junjie" sort="Xu, Junjie" uniqKey="Xu J" first="Junjie" last="Xu">Junjie Xu</name>
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<term>DNA, Viral (chemical synthesis)</term>
<term>Gene Library</term>
<term>Humans</term>
<term>Lentivirus (genetics)</term>
<term>Promoter Regions, Genetic</term>
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<term>Clonage moléculaire</term>
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<term>Cricetulus</term>
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<term>Clonage moléculaire</term>
<term>Cricetinae</term>
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<front><div type="abstract" xml:lang="en">Vector based shRNA (short hairpin RNA) expression library has been widely used to screen functional genes. For two main methods that have been used to generate short hairpin RNA libraries, chemical synthesis is too expensive to be widely used and the low efficiency of enzymatic approach makes it difficult to construct. We have developed a protocol to construct a new kind of shRNA library called randomized shRNA library. Within three steps chemically synthesized randomized 19-mers DNA were efficiently converted to double-stranded DNA fragments containing shRNA templates. This kind of shRNA library permits simple and economic construction, providing another choice for whole-genome phenotypic screening of genes.</div>
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Serveur d'exploration MERS

A randomized lentivirus shRNA library construction.

A randomized lentivirus shRNA library construction.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration MERS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.